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Analysis of fluG Mutations That Affect Light-Dependent Conidiation in Aspergillus nidulans

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Comparison of the effect of different wavelengths of light on conidiation and vegetative growth between fluG701 and wild-type strains. Strains TU 91 (fluG701; open bars) and WIM 126 (fluG + ; hatched bars) were incubated on complete medium at 32° in either full-spectrum white light (35 μE m −2 sec −1 ), red light (610 to 720 nm; 20 μE m −2 sec −1 ), blue light (436 nm; 5 μE m −2 sec −1 ), or the dark. (A) Conidial yield per colony was determined 24 hr after induction by the method of Y ager et al. ( 1982 ). N = 5 for all determinations; means are ± standard deviation. (B) N = 12 for all radial growth-rate determinations; means are ± standard deviation. An ANOVA analysis indicated no significant effect of genotype on growth in different illumination conditions (P > 0.05) and no significant difference of growth rate between allelic states (P > 0.05).

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Molecular identity of fluG mutations. (A) The 7-kb genomic region containing the fluG gene as defined by A dams et al. ( 1992 ) is shown. The hatched region and arrow indicate the transcription unit and direction of transcription. Uppercase letters indicate the location of the restriction enzymes sites for BamHI (B), EcoRI (E), HindIII (H), and XhoI (X). The indicated restriction fragments were cloned from plasmid pFM1 into pPK1 or pSM3 and transformed by the method of Y elton et al. ( 1984 ) into strains TU 39, TU 41, TU 42, and TU 136. The location of each mutation can be deduced by examining the smallest overlapping region among restriction fragments that complement each mutant phenotype. Lowercase letters indicate the location of each mutation: a, fluG10 sveA1 ; b, fluG701; c, fluG20 sveA1 ; and d, fluG30 sveA1 . (B) The predicted mutant regions were sequenced and compared to the wild type. Differences in nucleotide and predicted amino acid sequences are indicated. The numbered position of nucleotides and amino acids refers to the fluG sequence shown in L ee and A dams ( 1994a ).

Analysis of fluG Mutations That Affect Light-Dependent Conidiation in Aspergillus nidulans Find this author on Google Scholar Find this author on PubMed Search for this author