Categories
BLOG

strain central

M. tuberculosis Central Asian Strain 1 MDR isolates have more mutations in rpoB and katG genes compared with other genotypes

Affiliation

  • 1 Department of Pathology and Microbiology, The Aga Khan University, Karachi, Pakistan.
  • PMID: 19012077
  • DOI: 10.1080/00365540802570519

M. tuberculosis Central Asian Strain 1 MDR isolates have more mutations in rpoB and katG genes compared with other genotypes

  • Search in PubMed
  • Search in NLM Catalog
  • Add to Search

Authors

Affiliation

  • 1 Department of Pathology and Microbiology, The Aga Khan University, Karachi, Pakistan.
  • PMID: 19012077
  • DOI: 10.1080/00365540802570519

Erratum in

  • Scand J Infect Dis. 2010 Dec;42(11-12):953-4

Abstract

Pakistan ranks eighth globally among TB burden countries, with a MDR rate of 2-5%. The most prevalent MTB genotype is Central Asian Strain1 (CAS1) followed by the Beijing genogroup. We investigated common mutations in multidrug resistance encoding genes rpoB, katG and inhA of CAS1 and Beijing strains using DNA sequencing and fluorescent resonance energy transfer (FRET) probe based real-time PCR methods. 30 CAS1, 12 Beijing and 20 unclustered spoligotypes, and 10 susceptible MTB strains were tested. The most common mutations in the rpoB gene were at codons 531 (60%), 526 (23%) and 516 (5%). CAS1 strains had a higher frequency of mutations at codon 526 (p Takawira FT, Mandishora RSD, Dhlamini Z, Munemo E, Stray-Pedersen B. Takawira FT, et al. Pan Afr Med J. 2017 Jun 28;27:145. doi: 10.11604/pamj.2017.27.145.10883. eCollection 2017. Pan Afr Med J. 2017. PMID: 28904673 Free PMC article.

Pakistan ranks eighth globally among TB burden countries, with a MDR rate of 2-5%. The most prevalent MTB genotype is Central Asian Strain1 (CAS1) followed by the Beijing genogroup. We investigated common mutations in multidrug resistance encoding genes rpoB, katG and inhA of CAS1 and Beijing strain …